Expression of cathepsin L gene of fasciola gigantica in different developmental stages

Fascioliasis Is one of the most serious parasiiic infestations of livestock. It is caused by Fasciola hepatica (tremperate regions) and Fasciola gigantica (Tropf.cal reglons),It also has a zoonotic importance, the proteolytic enzyme: cathepsin-L cysteine proteinase (28-28,5 KDa) helps the parasite tn tissue invasion. host lmmune evasion and nutrients intake, This study aimed to check weather Fasciola gigantica flukes depend upon cathepsin-L throughout their life cycle in. snail and mammalian hosts or not, For that cuthepstn-L gene was investigated (n adult and other developmental stages of fasciola glgantica using semi-quantitative RT•PCR assay. Cathepsin-L protein was detected by using sodium dodecyl sulphate- polyacrylamide get electrophoresis [SDS-PAGE) analysts and detection of cathepsfn-L cysteine proteinase antigen in examined stages by Enzyme linked tmmunotransfer blot (EITP) analysts.